משפחה

CO-ADMINISRATION OF ORAL DOCOSAHEXAENOIC ACID AND INSULIN IN MICE – מתוך כנס גאסטרו ילדים, 9 ביולי 2002


O. Eshach Adiv, H. Mandel, N. Shehadeh, C. Knopf, Z. Shen Or, R. Shamir.

Rambam Medical center, Bruce Rappaport Faculty of Medicine, Haifa, Israel

Introduction: Insulin and docosahexaenoic acid (DHA) are present in human milk.

Docosahexaenoic acid (DHA) is a known intestinal absorptive enhancer for macromolecules like insulin, and increase insulin sensitivity. Our study aim was to examine the effect of co-administration of oral insulin and DHA in mice.

Methods: Immediately after weaning at 4 weeks of age, 28 BALB-C mice were divided into four groups for a period of 4 weeks: Group (GR) 1 did not receive insulin nor DHA, GR 2 received human insulin (1 unit/ml of drinking water) without DHA, GR 3 received DHA (500mg/kg/d in the chow diet) without insulin, and GR 4 was given human insulin and DHA. At 28 days, fasting blood levels of glucose, insulin, and lipids were performed. In addition, lipid peroxidation analysis, DHA plasma levels and DHA content in red blood cells were examined.

Results: Insulin levels in the insulin supplemented groups were significantly higher than those in the groups that were not supplemented (96.8לu/ml±45.5 Vs 0.64±0.03, P=0.001). Plasma levels of DHA were significantly higher in the DHA supplemented groups than those who were not (357.2לmol/l±36.2 Vs 464±20.3,p=0.02). Glucose levels were lower in GR 2 (61.4 mg/dL±2.8, p<0.0001) and in GR 3 (61.1 mg/dL±2.0, p<0.0001) compared to controls (71mg/dL±6.9). Supplementation of both insulin and DHA (GR 4) resulted in significantly lower glucose levels (56.4mg/dL±2.6 compared to GR 2 (p=0.004) and GR 3 (p=0.007). No significant differences were found in lipid levels or lipid peroxidation between the groups.

Conclusions: Supplementing insulin or DHA to the diet of weaned BALB-C mice reduces glucose blood levels. Supplementation of both has a synergistic effect. The presence of insulin and DHA in human milk may be the cause for reduced glucose levels in breast fed infants in addition to the known effects of DHA on insulin sensitivity

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